Km and Vmax: Michaelis-Menten equation

Km and Vmax: Michaelis-Menten equation. In this video, I have explained enzyme kinetics by taking varying concentrations of the substrate with constant enzyme concentration. Effect of varying concentrations of the substrate on the enzyme-catalyzed reaction in terms of rate of the reaction or velocity. By watching this video, you can understand the concept of Km, Vmax, initial velocity and Michaelis-Menten equation.

Factors affecting enzyme activity

In this video, I have explained various factors influencing the rate of enzyme-catalyzed reaction or enzyme activity. Effect of substrate concentration already discussed in the previous video. In this presentation, I have explained the effect of enzyme concentration, the effect of pH, and the effect of temperature on the velocity of enzyme-catalyzed reactions.

Lineweaver-Burk plot or double reciprocal plot

Lineweaver-Burk equation is nothing but reciprocal of the Michaelis-Menten equation and from this, we can easily calculate Km and Vmax. To know more about the Lineweaver-Burk plot please watch below embedded video.