Introduction and classification
Compound that binds to enzyme thereby decreases the activity of an enzyme or inhibits enzyme activity. To know more about enzyme inhibition types please watch below embedded video.
Competitive Enzyme Inhibition
In this video, I have explained the mechanism, kinetics examples and clinical significance of competitive enzyme inhibition. These competitive inhibitors of great help as they can be used as antibiotics, anticancer drugs, antihypertensive drugs, to treat gout, to treat hypercholesterolemia. common examples are allopurinol, malonate, methotrexate, dicumarol, captopril, enalapril, etc…
Noncompetitive Enzyme Inhibition
In this video, I have explained the definition, mechanism, enzyme kinetics and examples of non-competitive enzyme inhibition. Non-competitive enzyme inhibitors bind to an enzyme other than substrate binding site, thereby decreasing the rate of an enzyme-catalyzed reaction. Non-competitive enzyme inhibitors can bind both free enzyme and enzyme-substrate complex. In non-competitive enzyme inhibitor catalyzed reaction, there is no change in the Km value but a decrease in the Vmax or maximum velocity. Examples are cyanide, doxycycline, heavy metals like mercury and lead. 1:32 Mechanism of non-competitive enzyme inhibition 2:50 Enzyme inhibitor substrate complex 4:10 Enzyme kinetics graph of non-competitive enzyme inhibition, Km and Vmax 5:50 Lineweaver Burk plot or double reciprocal plot for non-competitive enzyme inhibition 7:05 Clinical significance or uses or application of non-competitive enzyme inhibitors 7:56 Cyanide as a non-competitive enzyme inhibitor 8:40 Heavy metals as a non-competitive enzyme inhibitor 9:50 Noncompetitive enzyme inhibitor examples etoposide, tacrine, trazodone. TF-505 and doxycycline
Uncompetitive Enzyme Inhibition
In this video, I have explained the mechanism of action, kinetics, and examples of Uncompetitive Enzyme Inhibition. In Uncompetitive Enzyme Inhibition, there are both decreased in the Km and Vmax.
Suicide Enzyme Inhibition
Suicide Enzyme Inhibition. In this video, I have explained the mechanism and clinical significance of suicide enzyme inhibition. 2:20 Normal enzyme action mechanism 2:47 Suicide enzyme inhibition mechanism, mechanism-based inactivation 5:04 Clinical significance or examples of suicide inhibitors, aspirin, Disulfiram, penicillin, deprenyl, 5-fluorouracil.